Efficacy of Methyl Cellosolve as a Cryoprotectant for Cryopreservation of Bovine Embryos
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چکیده
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Cryopreservation of common carp (Cyprinus carpio) embryos using different cryoprotectant
This study reports that common carp (Cyprinus carpio) embryos were successfully frozen and preserved by vitrification. The modified Haga solution was tested by using two different cryoprotectants and three different concentrations at 4°C to obtain appropriate vitrified solutions ((dimethylsulfoxide 5, 10, 15% (DMSO) and glycerol 5, 10, 15% (gly)) in the study. The hatching rates of embryos exp...
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15 صفحه اولinvestigating the feasibility of a proposed model for geometric design of deployable arch structures
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Title: 1 SHORT TERM CHILLED STORAGE OF ZEBRAFISH (Danio rerio) EMBRYOS IN 2 CRYOPROTECTANT AS AN ALTERNATIVE TO CRYOPRESERVATION
23 As zebrafish embryos never been cryopreserved, we developed a protocol to store zebrafish embryos 24 (50% epiboly5.3 hpf) for up to 18 h at 0° C. Initial experiments to optimise cryoprotectant solution 25 demonstrated improved embryo hatching rate following chilling at 0°C for 18 h with 1 M MeOH + 26 0.1 M sucrose (56 ± 5%) compared to other combination of methanol (0.2-, 0.5 M) and sucrose ...
متن کاملI-11: Cryopreservation of Canine Embryos
Background: The assisted reproductive techniques (ARTs) such as in vitro fertilization, embryo transfer and cryopreservation of gametes have considerably contributed to the development of biomedical sciences in addition to improved breeding in domestic animals and infertility treatment in humans. However, ARTs used in canine species have strictly limited utility when compared with other mammali...
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ژورنال
عنوان ژورنال: Journal of Reproduction and Development
سال: 1996
ISSN: 0916-8818,1348-4400
DOI: 10.1262/jrd.96-425j15